C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/131, 167/310
C12P 19/04 (2006.01) C12N 1/20 (2006.01) C12P 19/26 (2006.01)
Patent
CA 1336177
A novel mutant microorganism Streptococcus zooepidemi- cus HA-116 ATCC 39920, has been produced. The microor- ganism produces large amounts of high molecular weight hyaluronic acid. The invention provides a method of obtaining such microorganisms. The invention also concerns a method of obtaining sodi- um hyaluronate which comprises growing with vigorous agitation a microorganism of the genus Streptococcus under appropriate conditions in a suitable nutrient medium containing a sugar component as a carbon source. The sugar component is present in a substantially con- stant concentration between 0.2 and 10 grams per liter. The medium has a substantially constant pH between about 6.0 and 7.5 and includes a substantially constant magnesium ion concentration above 0.05 grams per liter. The sodium hyaluronate excreted into the medium by the organism is purified using methods involving precipa- tion, redissolving and reprecipating the hyaluronate. Composition of sodium hyaluronate which are character- ized by an absence of pyrogenicity and skin irritation are obtained.
499795
Greenman Benjamin
Kanner Dov
Landsberg Moshe
Nimrod Abraham
Bio-Technology General (israel) Ltd.
Gowling Lafleur Henderson Llp
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