Method of producing l-serine by fermentation

Details Method of producing l-serine by fermentation Method of producing l-serine by fermentation

C - Chemistry, Metallurgy

12

N

C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12P 13/06 (2006.01)

Patent

CA 2256758

L-serine is produced by cultivating in a medium a coryneform bacterium having L-serine productivity in which an activity of at least one of phosphoserine phosphatase and phosphoserine transaminase is enhanced, preferably, further having introduced therein a gene coding for D-3-phosophoglycerate dehydrogenase in which feedback inhibition by L-serine is desensitizied, allowing L-serine to accumulate in the medium, and collecting the L-serine from the medium.

De la L-sérine est produite par culture, dans un milieu, d'une corynébactérie possédant une productivité de L-sérine accompagnée d'un accroissement de l'activité de la phosphosérine phosphatase ou de la phosphosérine transaminase, ou des deux, idéalement après introduction d'un gène codant la D-3-phosophoglycérate déshydrogénase selon laquelle la rétroinihibition par la L-sérine est désensibilisée, ce qui permet à la L-sérine de s'accumuler dans le milieu et de recueillir la L-sérine du milieu.

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