Method of producing lys-plasminogen

Details Method of producing lys-plasminogen Method of producing lys-plasminogen

C - Chemistry, Metallurgy

12

N

195/127, 167/103

C12N 9/68 (2006.01) C12S 3/14 (2006.01) A61K 37/47 (1990.01)

Patent

CA 1334079

To produce lys-plasminogen having a specific activity of at least 17.5 caseinolytic units/mg protein and at least 50 µ moles/g protein nitrogen as well as an electrophoretic purity of at least 90 %, plasminogen from plasma, a plasminogen-containing fraction or a tissue culture is adsorbed on immobilized lysine for the purpose of purification, is eluted and is recovered from the eluate by a protein precipitating agent. A solution of the thus purified plasminogen is adjusted to a plasmin activity ranging between 0.005 and 0.2 µmoles/ml min relative to chromogenic substrate H-D-valyl-L-leucyl-L- lysine-p-nitroanilide dihydrochloride, is maintained at a temperature of from +1°C to +20°C for a period of from 6 to 60 hours in order to provoke an enzymatic-proteolytic conversion of plasminogen into lys-plasminogen, whereupon the enzymatic action is interrupted and lys-plasminogen is isolated.

606799

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