Method of producing peptides or proteins

Details Method of producing peptides or proteins Method of producing peptides or proteins

C - Chemistry, Metallurgy

12

P

C12P 21/06 (2006.01) C07K 14/31 (2006.01) C07K 14/575 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2070781

- 63 - ABSTRACT A fusion protein comprising a carrier and a de- sired peptide or protein linked to the carrier via an enzymatically excisable dipeptide, such as Lys-Arg, is produced in a host microorganism and then the desired peptide or protein is recovered by treating the fusion protein at least with a protease specifically recogniz- ing the dipeptide and specifically hydrolyzing the peptide bond of the dipeptide. The fusion protein includes tandem-form fusion proteins in which desired peptide or protein molecules are linked together re- peatedly. According to the cleavage site in the dipep- tide, the protease may be used in combination with an aminopeptidase specifically releasing a basic amino acid from the N-terminal side of the dipeptide and/or a carboxypeptidase specifically releasing a basic amino acid from the C-terminal side of the dipeptide.

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