C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01)
Patent
CA 2321965
A method of making plants which are resistant or tolerant to herbicides which, in vitro, inhibit 4-hydroxyphenylpyruvate dioxygenase (4HPPD) comprises the steps of: (i) transforming plant material with a polynucleotide comprising a region encoding a phytoene desaturase; (ii) regenerating the thus transformed material into morphologically normal plants. In a preferred embodiment the region comprised by the polynucleotide is the sequence depicted in SEQ ID No.1, or is a sequence which is complementary to one which when incubated at a temperature of between 55 and 60 ~C in 0.3 strength citrate buffered saline containing 0.1 % SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1 % SDS still hybridises with the sequence depicted in SEQ ID No.1.
L'invention concerne un procédé de production de plantes tolérantes ou résistantes aux herbicides et qui, in vitro, inhibent 4-hydroxyphénylpyruvate-dioxygénase (4HPPD). Le procédé consiste à i) transformer un matériel végétal comprenant un polynucléotide dont une région code un phytoène-désaturase; ii) régénérer le matériel ainsi transformé en plantes à morphologie normale. Dans une forme de réalisation préférée, cette région incluse dans le polynucléotide représente la séquence décrite dans SEQ ID No.1 ou est une séquence complémentaire à une autre séquence qui, une fois incubée à une température comprise entre 55 et 60 ·C dans un soluté tampon de citrate d'une teneur de 0,3 contenant 0,1 % de SDS et après rinçage dans les mêmes conditions, s'hybride toujours avec ladite séquence décrite dans SEQ ID No.1.
Bryan Ian Bennett
Shipton Catherine Ann
Na
Zeneca Limited
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