Method of producing polymers of spider silk proteins

C - Chemistry – Metallurgy – 07 – K

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C07K 14/435 (2006.01) D01F 4/00 (2006.01)

Patent

CA 2759465

A method of producing polymers of an isolated spider silk protein involves providing a solution of said spider silk protein in a liquid medium at p H 6.4 or higher and/or an ion composition that prevents polymerisation of the spider silk protein. The properties of the liquid medium are adjusted to a pH of 6.3 or lower and an ion composition that allows polymerisation of the spider silk protein. The spider silk protein is allowed to form polymers in the liquid medium, and the resulting spider silk protein polymers are isolated from the liquid medium. The resulting polymers are useful as fibers, films, foams, nets or meshes.

L'invention porte sur un procédé de production de polymères d'une protéine de soie d'araignée isolée, lequel procédé met en jeu les opérations consistant à se procurer une solution de ladite protéine de soie d'araignée dans un milieu liquide à pH 6,4 ou plus et/ou à une composition ionique qui empêche une polymérisation de la protéine de soie d'araignée. Les propriétés du milieu liquide sont ajustées à un pH de 6,3 ou moins et à une composition ionique qui permet la polymérisation de la protéine de soie d'araignée. La protéine de soie d'araignée est amenée à former des polymères dans le milieu liquide, et les polymères de protéine de soie d'araignée résultants sont isolés du milieu liquide. Les polymères résultants sont utiles en tant que fibres, films, mousses, filets ou mailles.

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