Method of producing recombinant dna molecules

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2529246

The present invention is directed to an improved method for producing by recombinant methods proteins that occur in nature in two or more subunits; more generally, it is applicable to the amplification and subsequent expression of any chimeric DNA molecule that results form the ligation of two or more non-contiguous pieces of DNA.

La présente invention concerne une méthode améliorée destinée à produire, au moyen de méthodes de recombinaison, des protéines existant dans la nature dans deux ou plusieurs sous-unités. Plus généralement, l'invention s'applique à l'amplification et à l'expression subséquente d'une quelconque molécule d'ADN chimérique résultant de la ligature de deux ou plusieurs parties non contiguës d'ADN.

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