Method of producing recombinant proteins in the mammary...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) C12N 15/85 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2502902

The invention relates to a method of producing proteins of biopharmaceutical interest from the mammary gland of non-transgenic animals. The genes which code for said proteins are transferred to the mammary epithelial cells using adenoviral vectors. Such vectors can be based on adenoviruses with defective replication, such as .DELTA.E1.DELTA.E3, or on adjuvant-dependent adenoviruses from which all or almost all of the adenoviral coding sequences have been eliminated. With said method, high levels of recombinant proteins can be produced in milk, thereby enabling the large-scale production of proteins of biopharmaceutical interest in the biologically active form thereof.

L'invention concerne une méthode de production de protéines d'intérêt bio-pharmaceutique à partir de la glande mammaire d'animaux non transgéniques. Les gènes codant pour lesdites protéines sont transférés dans les cellules épithéliales mammaires à l'aide de vecteurs adénoviraux. Ces vecteurs peuvent être basés sur des adénovirus à réplication défectueuse comme le ?E1?E3, ou des adénovirus dépendant d'un assistant dans lesquels ont été éliminées toutes ou presque toutes les séquences adénovirales de codification. Cette méthode permet d'obtenir des niveaux élevés de protéines de recombinaison dans le lait, permettant ainsi la production à grande échelle de protéines d'intérêt bio-pharmaceutique dans leur forme biologiquement active.

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