Method of producing recombinant tat-hoxb4h protein for use...

Details Method of producing recombinant tat-hoxb4h protein for use... Method of producing recombinant tat-hoxb4h protein for use...

C - Chemistry, Metallurgy

07

K

C07K 19/00 (2006.01) A61K 38/16 (2006.01) C07K 1/02 (2006.01) C07K 1/14 (2006.01) C07K 1/22 (2006.01) C07K 14/16 (2006.01) C07K 14/47 (2006.01) C12N 15/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2658231

The present invention relates to a new and nonobvious method of producing the C-terminal histidine tagged TAT-HOXB4 fusion protein (TAT-HOXB4H), providing unexpected benefits of increased yield and stability to allow for in vivo administration of this protein, and pharmaceutical composition comprising an effective ingredient, TAT-HOXB4H, having stimulatory activity on the production of hematopoietic cells. More specifically, recombinant TAT-HOXB4H protein enhances engraftment of bone marrow transplants, hematopoietic reconstruction, bone marrow re-population and number of circulating stem cells, particularly after chemotherapy or irradiation.

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