Method of producing rooted cuttings of tree belonging to the...

A - Human Necessities – 01 – G

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A01G 1/00 (2006.01)

Patent

CA 2690346

The present invention provides a method for producing a rooted cutting which can be applied to Pinaceae trees. Specifically, the present invention provides a method for producing a rooted cutting of a Pinaceae tree, comprising the following steps A and B. A: adding a cytokinin to the outside of a shoot apex of a short shoot of a Pinaceae tree to thereby induce a new scaly leaf and/or primary leaf; B: cutting out the short shoot where new scaly leaf and/or primary leaf is induced in the step A, inserting the short shoot as a cutting in a rooting bed wetted with a carbon source-free liquid medium comprising nitrogen, phosphorus and potassium as essential elements, and culturing the short shoot in a culturing vessel while regulating the concentration of CO2 gas in the culturing vessel at a humidity of 80% or more to thereby root the cutting.

L'invention vise à proposer un procédé de production de boutures racinées qui est applicable à un arbre appartenant à la famille des Pinaceae. Plus précisément, l'invention propose un procédé de production de boutures racinées d'un arbre appartenant à la famille des Pinaceae qui comporte les étapes A et B suivantes. A) L'addition externe d'une cytokinine à l'apex de pousse de courtes branches d'un arbre appartenant à la famille des Pinaceae et ainsi incitant la formation de nouvelles feuilles et/ou de feuilles primaires. B. L'émondage de courtes branches dans lesquelles de nouvelles feuilles et/ou des feuilles primaires ont été formées dans l'étape A ci-dessus, l'enfoncement de ces branches émondées, qui sont employées en tant que boutures, dans un lit d'enracinement ayant été humidifié par un milieu liquide contenant de l'azote, du phosphore et du potassium comme éléments essentiels mais étant exempt d'une source de carbone, et la mise en culture dans un contenant de culture à une humidité d'au moins 80 % tout en régulant la concentration en dioxyde de carbone gazeux dans le contenant de culture, permettant ainsi aux boutures de s'enraciner.

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