C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/08 (2006.01) C07K 16/00 (2006.01) C07K 16/44 (2006.01) G01N 33/577 (2006.01)
Patent
CA 2197232
The invention relates to a method of producing single-chain Fv molecules in eukaryotic cells, and to secretable sFv proteins having at least one non- naturally occurring glycosylation site. The single-chain Fv molecules produced by this method are biologically active and capable of being secreted from eukaryotic cells into the cell culture medium.
L'invention porte sur un procédé de production de molécules Fv à chaîne unique dans des cellules eucaryotes, et sur des protéines sFv sécrétables comportant au moins un site de glycosylation non naturel. Les molécules Fv à chaîne unique obtenues selon ce procédé sont biologiquement actives et peuvent être sécrétées par des cellules eucaryotiques dans le milieu de culture cellulaire.
Huston James S.
Jost Carolina R.
Segal David M.
Borden Ladner Gervais Llp
Creative Biomolecules Inc.
The Government Of The United States Of America As Represented By Department Of Health And Human Services
United States Of America Represented By The Secretary Of The Department Of Health And Hum
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