Method of producing uridine 5'-diphospho-n-acetylgalactosamine

Details Method of producing uridine 5'-diphospho-n-acetylgalactosamine Method of producing uridine 5'-diphospho-n-acetylgalactosamine

C - Chemistry, Metallurgy

12

P

C12P 19/30 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2582686

A method of producing uridine 5~-diphospho-N-acetylgalactosamine (UDP-GalNAc), which is an important substrate in synthesizing an oligosaccharide, characterized in that in enzymatically producing uridine 5~-diphospho-N- acetylgalactosamine from uridine 5~-triphosphate (UTP) and N- acetylgalactosamine-1-phosphate (GalNAc1-P), uridine 5~-diphospho-N- acetylgalactosamine pyrophosphorylase (UDP-GlcNAc pyrophosphorylase) originating in a microorganism (excluding pathogenic ones) is used as an enzyme. In this method, it is also possible to use, as GalNAc-1P, a product prepared from N-acetylgalactosamine and a phosphate donor in a reaction system with the use of N-acetylgalactosamine kinase. According to this method, uridine 5~-diphospho-N-acetylgalactosamine can be efficiently produced by using a relatively inexpensive substrate.

Cette invention concerne un procédé de production d'uridine 5'-diphospho-N-acétylgalactosamine (UDP-GalNAc) qui constitue un substrat important dans la synthèse d'un oligosaccharide, lequel procédé se caractérise en ce que la production enzymatique d'uridine 5'-diphospho-N-acétylgalactosamine à partir d'uridine 5'-triphosphate (UTP) et de N-acétylgalactosamine-1-phosphate (GalNAc1-P) entraîne la génération, dans un micro-organisme (à l'exception des micro-organismes pathogènes), d'uridine 5'-diphospho-N-acétylgalactosamine pyrophosphorylase (UDP-GlcNAc pyrophosphorylase) qu'on utilise comme enzyme. Dans ce procédé, on peut également utiliser, comme GalNAc-1P, un produit préparé à partir de N-acétylgalactosamine et d'un donneur de phosphate dans un système réactionel à l'aide de N-acétylgalactosamine kinase. Selon ce procédé, on peut produire efficacement de l'uridine 5'-diphospho-N-acétylgalactosamine à l'aide d'un substrat relativement peu coûteux.

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