Method of profiling a cell population

Details Method of profiling a cell population Method of profiling a cell population

G - Physics

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N

G01N 33/50 (2006.01) C07H 3/06 (2006.01) C12Q 1/68 (2006.01) G01N 33/567 (2006.01) G01N 33/577 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2650841

The present invention relates to a method of profiling a cell population comprising a step of detecting the presence or absence of at least two biological markers in said cell population, wherein at least one of said markers is a cell surface marker, which is a sialylated N-glycan marker with structure NeuNAc.alpha.3Gal, and at least one of said markers is a mRNA marker related to glycoproteins and/or glycosynthase proteins. The invention also relates to method for purification of cord blood cell population and to a complete cell population from cord blood purified by said method.

L'invention concerne un procédé de profilage d'une population cellulaire qui consiste à détecter la présence ou l'absence d'au moins deux marqueurs biologiques dans ladite population cellulaire, au moins un marqueur étant un marqueur de surface cellulaire, qui est un marqueur N-glycane sialylé de structure NeuNAca3Gal, et au moins un desdits marqueurs étant un marqueur mARN lié à des glycoprotéines et/ou à des protéines de la glycosynthase. L'invention concerne également un procédé de purification de la population cellulaire du sang ombilical et un procédé d'obtention d'une population cellulaire du sang ombilical purifiée au moyen dudit procédé.

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