Method of prognosis of metastasis by detection of fra12e...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2550429

Provided are methods for prognostic prediction of metastasis. The method comprises identifying the presence or absence of a fragile site FRA12E on chromosome 12q24 at the SMRT gene locus. The presence of FRA12E in an individual can be determined in cell samples obtained from the individual by hybridization techniques such as fluorescence in situ hybridization techniques. The presence of this fragile site at this locus is indicative of higher likelihood of metastasis than if the fragile site is absent. Also provided are kits for the determination of likelihood of metastasis.

L'invention concerne des méthodes de prédiction pronostique de métastase. La méthode consiste à identifier la présence ou l'absence d'un site fragile de FRA12E sur le chromosome 12q24 au niveau du locus du gène SMRT. La présence de FRA12E chez une personne peut être déterminée dans des échantillons cellulaires prélevés chez la personne par des techniques d'hybridation, telles que des techniques d'hybridation in situ à fluorescence. La présence du site fragile au niveau de ce locus indique une probabilité supérieure de métastase par rapport au cas où ledit site fragile est absent. L'invention porte également sur des kits de détermination de la probabilité de métastase.

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