Method of proliferating human hepatocytes and method of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/071 (2010.01) A61K 35/407 (2006.01) A61L 27/00 (2006.01) A01K 67/027 (2006.01) C12N 5/18 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2480561

Human hepatocytes are transplanted into the liver of a mouse suffering from immunodeficient hepatopathy and then the mouse carrying the human hepatocytes transplanted therein is fed under such conditions as being protected from the attack by a human complement produced by the human hepatocytes. Thus, the transplanted human hepatocytes are proliferated on a mass scale in the mouse liver. By repeating the above procedure with the use of the human hepatocytes thus proliferated, human hepatocytes are obtained in a large amount.

Des hépatocytes humains sont transplantés dans le foie d'une souris souffrant d'une hépatopathie associée à une immunodéficience, puis la souris porteuse des hépatocytes humains transplantés est alimentée dans des conditions assurant sa protection contre l'attaque par un complément humain produit par les hépatocytes humains. Ainsi, les hépatocytes humains transplantés prolifèrent à grande échelle dans le foie de la souris. Il est possible d'obtenir en grande quantité d'hépatocytes humains en répétant la procédure de prolifération d'hépatocytes humains décrite ci-dessus.

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