Method of proliferating natural killer cells

Details Method of proliferating natural killer cells Method of proliferating natural killer cells

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) A61K 35/26 (2006.01) A61K 39/00 (2006.01) A61P 35/00 (2006.01) C12Q 1/02 (2006.01) A61K 35/12 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2393322

A method of proliferating natural killer cells involving the step of culturing peripheral blood mononuclear cells together with a human Wilms' tumor cell line HFWT. Natural killer cells of a human individual can be proliferated by, for example, using peripheral blood mononuclear cells collected from the human individual per se. Thus, natural killers sustaining a high cytotoxic activity can be efficiently proliferated.

Une méthode de prolifération de cellules tueuses naturelles comprend l'étape consistant à mettre en culture des cellules mononucléaires du sang périphérique avec une lignée cellulaire de tumeur de Wilm HFWT. Les cellules tueuses naturelles d'un individu humain peuvent être mises à proliférer, par exemple, à l'aide de cellules mononucléaires de sang périphérique prélevé sur l'individu humain per se. Ainsi, des cellules tueuses naturelles entretenant une activité cytotoxique élevée peuvent être mises à proliférer efficacement.

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