Method of protein extraction from cells

Details Method of protein extraction from cells Method of protein extraction from cells

C - Chemistry, Metallurgy

07

K

C07K 1/14 (2006.01) C07K 14/005 (2006.01) C07K 14/025 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2652118

Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.

La présente invention a pour objet des méthodes de production d'un extrait protéinique à partir de cellules, par exemple de cellules contenant des protéines virales. Les méthodes impliquent globalement : l'augmentation du pH des cellules jusqu'à une valeur de pH d'au moins environ 10,0 pour produire une composition intermédiaire, puis, en présence d'un détergent non ionique, la neutralisation du pH de la composition intermédiaire pour obtenir l'extrait protéinique. La présente invention concerne également des kits et des compositions permettant l'application des méthodes selon l'invention.

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