Method of protein purification

Details Method of protein purification Method of protein purification

C - Chemistry, Metallurgy

07

K

C07K 1/22 (2006.01)

Patent

CA 2467048

Purification methods are provided for proteins and peptides, employing silicon carbide to bind the proteins or peptides. The methods may also be used to recover and purify recombinantly expressed proteins sequestered in inclusion bodies. The method for purifying a protein or peptide comprises contacting a solution containing the protein or peptide with silicon carbide at a binding pH for the protein or peptide to allow the protein or peptide to bind to the silicon carbide; and eluting the protein or peptide from the silicon carbide.

L'invention concerne des procédés de purification de protéines et de peptides faisant appel à du carbure de silicium pour fixer les protéines ou les peptides. Ces procédés peuvent également être mis en oeuvre pour la récupération et la purification de protéines exprimées par recombinaison, séquestrées dans des corps d'inclusion. Un tel procédé de purification d'une protéine ou d'un peptide comprend les étapes suivantes: mise en contact d'une solution contenant la protéine ou le peptide avec du carbure de silicium à un pH permettant la liaison de la protéine ou du peptide carbure de silicium; et élution de la protéine ou du peptide du carbure de silicium.

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