Method of purification of amylase, purified amylase so...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/28 (2006.01) A61K 38/47 (2006.01) C07K 1/30 (2006.01) C11D 3/386 (2006.01)

Patent

CA 2108699

The present invention relates to a method for the preparation of a purified amylase recovered from a fermentation broth, comprising the addition of a precipitation agent which is a halide salt, most preferably sodium or potassium chloride. According to a preferred embodiment, a further precipitation agent is added which is an organic compound, most preferably an alkyl ester of 4-hydroxybenzoic acid. The present invention also relates to the purified amylases so obtained and to the uses of these purified amylases.

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