Method of purification of recombinant viral vectors...

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G01N 15/10 (2006.01) C12Q 1/70 (2006.01) G01N 21/31 (2006.01) G01N 30/96 (2006.01) C12N 7/02 (2006.01) C12N 15/86 (2006.01) C12Q 1/24 (2006.01)

Patent

CA 2522630

The invention provides a method of determining the number of intact viral particles in a sample, the method comprising: (a) chromatographing the sample containing the intact viral particles on an anion exchange resin; (b) monitoring the absorbance of the eluate from the chromatography of step (a) at a selected wavelength; and (c)determining the total number of intact viral particles in the sample by comparing the absorbance value obtained in step (b) to a standard curve which relates absorbance to number of viral particles.

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