Method of purifying calcium ion-binding protein

Details Method of purifying calcium ion-binding protein Method of purifying calcium ion-binding protein

C - Chemistry, Metallurgy

07

K

C07K 1/18 (2006.01) C07K 2/00 (2006.01) C07K 14/47 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2385371

A method of purifying a calcium ion-binding protein by the cation exchange method whereby the calcium ion-binding protein is conveniently and efficiently separated and purified from a liquid containing the calcium ion-binding protein and contaminants without resort to any pretreatment of, for example, adding a chelating agent. More particularly, the method comprises, in the process of purifying a calcium ion-binding protein, adsorbing the protein by a cation exchanger in the presence of calcium ion, washing and then eluting. A calcium ion-binding protein obtained by this method which is substantially free from any impurities.

La présente invention concerne un procédé de purification de la protéine de liaison à l'ion calcium par la voie de l'échange cationique dans lequel la protéine de liaison à l'ion de calcium est séparé de manière appropriée et efficace d'un liquide contenant la protéine de liaison à l'ion calcium et des contaminants sans recourir à un prétraitement, par exemple l'ajout d'un agent complexant. Plus particulièrement, le procédé comporte, dans la phase de purification de la protéine de liaison à l'ion calcium, une adsorption de la protéine par un échangeur de cation en présence de l'ion calcium, un lavage et une élution. L'invention concerne également une protéine de liaison à l'ion calcium exempte d'impuretés obtenue par ledit procédé.

Affiliated with

Also associated with

Rating

Method of purifying calcium ion-binding protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of purifying calcium ion-binding protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of purifying calcium ion-binding protein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1880747