C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) B01D 21/26 (2006.01) C07H 1/08 (2006.01)
Patent
CA 2277468
The invention concerns a method of purifying extrachromosomal DNA by passing an extrachromosomal DNA and fluid containing further cell components through a cross-flow centrifuge under given conditions, resulting in separation of the extrachromosomal DNA from the other cell components such that purified extrachromosomal DNA is obtained. The invention further concerns the use of the purified extrachromosomal DNA for cloning, transformation, transfection and microinjection into cells, for use in gene therapy processes, DNA vaccination and/or for polymerase chain reaction (PCR). The invention finally concerns the use of a cross-flow centrifuge for purifying extrachromosomal DNA.
L'invention concerne un procédé de purification d'ADN extrachromosomal, par guidage à travers une centrifugeuse dans des conditions données, d'un ADN extrachromosomal et d'un liquide contenant d'autres constituants cellulaires, ce qui entraîne la séparation de l'ADN extrachromosomal des autres constituants cellulaires, de manière à obtenir l'ADN extrachromosomal purifié. L'invention concerne en outre l'utilisation de l'ADN extrachromosomal à des fins de clonage, de transformation, de transfection, de micro-injection dans des cellules, d'application dans des procédés de thérapie génique, de vaccination par ADN et/ou de réaction en chaîne de la polymérase (PCR). L'invention concerne par ailleurs une centrifugeuse tangentielle pour purifier de l'ADN extrachromosomal.
Kuhne Wolfgang
Popp Friedrich
Gowling Lafleur Henderson Llp
Roche Diagnostics Gmbh
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