Method of purifying hepatitis a virus particles and vaccine...

A - Human Necessities – 61 – K

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A61K 39/29 (2006.01) A61K 39/295 (2006.01) C12N 7/02 (2006.01)

Patent

CA 2468297

The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a preparation consisting of purified mature HAV particles in an amount sufficient to induce a protective immune in a mammal.

La présente invention concerne des méthodes de purification du virus de l'hépatite A du surnageant d'une culture cellulaire infectée et de production d'une préparation d'antigène purifié du virus de l'hépatite A. La présente invention concerne également une composition vaccinale contre le virus de l'hépatite A comprenant une préparation constituée de particules mûres du virus de l'hépatite A en quantité suffisante pour induire une réponse immunitaire protectrice chez un mammifère.

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