Method of purifying heterologous proteins

Details Method of purifying heterologous proteins Method of purifying heterologous proteins

C - Chemistry, Metallurgy

12

N

C12N 15/00 (2006.01) A01K 67/00 (2006.01) C07K 14/00 (2006.01) C12N 15/09 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2371941

A method of separating a heterologous protein expressed by a host cell, e.g., expressed by a cell in a transgenic mammal, and the version of the protein naturally expressed by the host cell is disclosed. The method includes providing a cell, e.g., a cell in a transgenic mammal, which includes a sequence encoding the heterologous polypeptide and which expresses an altered form of the protein naturally expressed by the cell, e.g., the cell of a transgenic mammal.

Cette invention concerne un procédé permettant de séparer une protéine hétérologue exprimée par une cellule hôte, c'est-à-dire exprimée par une cellule de mammifère transgénique, ainsi que la version de la protéine exprimée naturellement par la cellule hôte. Ce procédé s'applique à une cellule, telle qu'une cellule d'animal transgénique, qui renferme une séquence codant pour le polypeptide hétérologue et qui exprime une forme altérée de la protéine exprimée naturellement par la cellule, c'est-à-dire la cellule d'un mammifère transgénique.

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