Method of purifying protein from inclusion bodies

Details Method of purifying protein from inclusion bodies Method of purifying protein from inclusion bodies

C - Chemistry, Metallurgy

07

K

C07K 1/113 (2006.01) C07K 1/36 (2006.01) C07K 14/50 (2006.01) C07K 14/52 (2006.01) C07K 16/24 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2267641

The present invention relates to processes for the purification of proteins. More specifically, methods for solubilizing and purifying proteins expressed in an insoluble form using low concentrations of chaotropic agents, such as guanidine salts, are provided. Also provided are methods for refolding proteins solubilized according to the present invention.

La présente invention se rapporte à des procédés de purification de protéines. Elle se rapporte plus particulièrement à des procédés de solubilisation et de purification de protéines exprimées sous forme insoluble, qui font usage de faibles concentrations d'agents chaotropiques, tels que des sels de guanidine. L'invention se rapporte également à des procédés de renaturation de protéines solubilisées conformément aux procédés décrits ci-dessus.

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