Method of purifying toxin conjugates using hydrophobic...

Details Method of purifying toxin conjugates using hydrophobic... Method of purifying toxin conjugates using hydrophobic...

C - Chemistry, Metallurgy

07

K

167/129, 530/3.2

C07K 17/00 (2006.01) A61K 47/48 (2006.01)

Patent

CA 1305415

Abstract of the Disclosure A method of isolating and purifying toxin conjugates using hydrophobic interaction chromatography. Crude conjugate mixtures are sized to remove unconjugated toxin, and loaded onto a column filled with a suitable hydrophobic gel. Elution is effected with salt solutions of decreasing ionic strength, which salt solutions optionally include increasing amounts of an organic solvent. Toxin conjugate substantially free of unconjugated Ig and unconjugated toxin is provided.

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