Method of quantifying the surfactant of a protein preparation

G - Physics – 01 – N

Patent

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G01N 31/22 (2006.01) C07K 1/30 (2006.01) C11D 1/00 (2006.01) G01N 21/78 (2006.01)

Patent

CA 2524630

The present invention provides a method for removing a proteinaceous component from a liquid-phase surfactant preparation comprising (a) providing a liquid- phase surfactant preparation containing a proteinaceous component; (b) adding a complexing agent to the preparation of step (a) and allowing the complexing agent to form a complex with the surfactant; (c) simultaneously with step (b), or subsequently, adding a miscible precipitating agent to the preparation of step (a) or the product of step (b), io respectively, to form a liquid-phase reaction mixture and allowing the miscible precipitating agent to precipitate the proteinaceous component within the liquid-phase reaction mixture; and (d) separating the said complex from the precipitated proteinaceous component in the product of step (c) to provide a purified liquid-phase surfactant preparation; wherein the complex remains in solution within the liquid-phase reaction mixture, and wherein step (d) retains the complex in the liquid phase.

L'invention concerne une méthode servant à extraire un élément protéiné d'une préparation tensioactive en phase liquide, ce qui consiste à : (a) mettre en application une préparation tensioactive en phase liquide contenant un élément protéiné ; (b) ajouter un agent séquestrant à cette préparation (a) et permettre à l'agent séquestrant de constituer un complexe avec le tensioactif ; (c) simultanément ou consécutivement à l'étape (b), ajouter un agent de précipitation miscible à la préparation de l'étape (a) ou au produit de l'étape (b), de manière à obtenir un mélange réactionnel en phase liquide et permettre à cet agent de précipitation miscible de précipiter l'élément protéiné à l'intérieur du mélange réactionnel en phase liquide ; (d) séparer ledit complexe dudit élément protéiné précipité dans le produit de l'étape (c) afin d'obtenir une préparation tensioactive en phase liquide purifiée, le complexe restant en solution à l'intérieur du mélange réactionnel en phase liquide et l'étape (d) retenant ce complexe en phase liquide.

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