Method of quantitation by mass spectrometry

G - Physics – 01 – N

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G01N 27/00 (2006.01) H01J 49/26 (2006.01)

Patent

CA 2713082

Quantitation is performed using data from a mass spectrometer. A calibration ion mass spectrum is acquired for each of a plurality of known quantities of a material. From the calibration spectra a plurality of ions that identify the material is determined, and for each ion of the plurality of ions a linear range and linear function are determined. A sample ion mass spectrum is acquired for an unknown quantity of the material. A sample intensity is measured for each ion of the plurality of ions from the sample spectrum. After acquiring the sample spectrum, one or more ions are selected from the plurality of ions such that the sample intensity of each of the one or more ions is within a linear range of the ion. The unknown quantity is calculated from the sample intensities and linear functions of the one or more ions.

Linvention concerne une quantification effectuée à laide de données provenant dun spectromètre de masse. Un spectre de masse dions détalonnage est acquis pour chacune des quantités dune pluralité de quantités connues dune matière. A partir des spectres détalonnage, une pluralité dions, qui identifient la matière, est déterminée, et pour chaque ion de la pluralité dions, une plage linéaire ou fonction linéaire est déterminée. Un spectre de masse dions déchantillon est acquis pour une quantité inconnue de la matière. Une intensité déchantillon est mesurée pour chaque ion de la pluralité dions à partir du spectre déchantillon. Après acquisition du spectre déchantillon, un ou plusieurs ions sont choisis parmi la pluralité dions, de telle sorte que lintensité déchantillon de chaque ion ou de chacun des ions se trouve dans une plage linéaire de lion. La quantité inconnue est calculée à partir des intensités déchantillon et des fonctions linéaires de lion ou des ions.

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