Method of quantitative determination of bilirubin and a...

Details Method of quantitative determination of bilirubin and a... Method of quantitative determination of bilirubin and a...

G - Physics

01

N

G01N 33/72 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2188618

Disclosed are a method for quantitative determination of bilirubin and a reagent for the method, which are useful in clinical examinations. The method comprises subjecting the bilirubin in a sample to coexist with ascorbate oxidase and a reaction promoter in aqueous medium to thereby oxidize the bilirubin, measuring the change in absorbance of the aqueous medium and comparing the change in absorbance with a calibration curve. The reagent comprises ascorbate oxidase and a reaction promoter.

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