Method of quantitative determination of peroxide, a...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/28 (2006.01) C07D 471/14 (2006.01) G01N 31/22 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2177456

Disclosed is a chemiluminescent method of quantitatively determining one of peroxide, a peroxidation-active substance or a pyrazolopyridopyridazine derivative represented by the formula (I): Image (I) wherein R1 represents hydrogen, lower alkyl, optionally substituted aryl, optionally substituted heterocyclic group, substituted sulfonyl, substituted sulfinyl, substituted thio etc.; R2 represents hydrogen, lower alkyl, optionally substituted aryl, etc., or a salt thereof; characterized by subjecting an unknown amount of one substance selected from the peroxide, peroxidation-active substance and pyrazolopyridopyridazine derivative to react with a known amount of the remaining two substances.

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