Method of quantitative determination utilizing enzyme...

Details Method of quantitative determination utilizing enzyme... Method of quantitative determination utilizing enzyme...

G - Physics

01

N

167/45

G01N 33/58 (2006.01) C12Q 1/26 (2006.01) G01N 33/542 (2006.01) G01N 33/563 (2006.01)

Patent

CA 1208550

METHOD OF QUANTITATIVE DETERMINATION UTILIZING ENZYME AGGREGATION ABSTRACT OF THE DISCLOSURE The invention provides a method for the quan- titative determination of an analyte in a sample. The method involves providing a sample of an analyte having at least two receptor binding sites, providing a conjugate comprising one molecule of an enzyme and one or more of a receptor, wherein the conjugate is capable of binding to the analyte. The enzyme of the conjugate is thereby linked by bonding among receptor-analyte-receptor so that the enzyme activity changes according to the amount of analyte present. By measuring the activity of the enzyme, a quantitative determination of the analyte can be made.

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