Method of recovering lipase activity

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/20 (2006.01) C11C 3/02 (2006.01) C11C 3/10 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2652076

Disclosed is a method for recovery of a lipase activity, which comprises subjecting a lipase powder composition to an esterification or transesterification reaction and then washing the composition with triacyl glycerol, wherein the lipase powder composition comprises: a lipase derived from a microorganism belonging to the genus Thermomyces which is immobilized on a carrier, or a pulverized product of the lipase which has an average particle diameter of not smaller than 1 µm and smaller than 300 µm and is immobilized on a carrier; and a filter aid. The method enables to recover a decreased activity of a lipase with high efficiency.

La présente invention concerne un procédé de régénération de l'activité d'une lipase, ledit procédé impliquant de soumettre une composition de lipase en poudre à une réaction d'estérification ou de transestérification, puis de laver la composition avec du triacylglycérol. Ladite composition de lipase en poudre comprend : une lipase dérivée d'un micro-organisme appartenant au genre Thermomyces immobilisée sur un support, ou un produit pulvérisé dérivé de la lipase ayant un diamètre moyen de particule supérieur ou égal à 1 µm et inférieur à 300 µm immobilisé sur un support ; et un auxiliaire de filtration. Le procédé permet de restaurer avec une efficacité élevée l'activité d'une lipase qui avait diminué.

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