Method of recovering microbially produced recombinant ricin...

Details Method of recovering microbially produced recombinant ricin... Method of recovering microbially produced recombinant ricin...

C - Chemistry, Metallurgy

07

K

195/73, 530/15.0

C07K 14/415 (2006.01)

Patent

CA 1308680

Abstract of the Disclosure Substantially pure, intracellularly produced, soluble recombinant ricin toxin A (RTA) is recovered from transformed cells by disrupting the cell membrane, removing insoluble cell membrane materials from the disruptate, adjusting the pH of the cell membrane material-free solution to 6 to 6.5 and the conductivity to 1.25 to 1.75 millisiemens, passing the adjusted solution through a bed of SP- cellulose cation exchanger, and eluting the substantially pure RTA from the bed.

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