Method of reducing scattered light during photometric...

Details Method of reducing scattered light during photometric... Method of reducing scattered light during photometric...

G - Physics

01

J

150/1

G01J 1/00 (2006.01) G01N 21/59 (2006.01) G01N 33/483 (2006.01)

Patent

CA 1115182

INVENTOR: RAYMOND FREY INVENTION: METHOD OF REDUCING SCATTERED LIGHT DURING PHOTO- METRIC MEASUREMENT, ESPECIALLY PHOTOMETRIC MEASUREMENT OF HAEMOGLOBIN AND REAGENT FOR PERFORMANCE OF THE METHOD ABSTRACT OF THE DISCLOSURE A method of reducing scattered light during photometric measurements, especially during the photometric measurement of haemoglobin and a reagent for the performance of the method is disclosed. At a suspension which is ready for a measurement operation there is admixed an additive which reduces the differ- ence between the refractive index of the suspension and the mean refractive index of the suspended phase. The reagent for the photometric determination of the haemoglobin comprises a lysising agent, means for modifying the refractive index, means for stabilizing the leukocytes and a solvent. The reagent is admixed as an additive to a suspension of blood particles in an isotonic solvent.

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