Method of regenerating rice plant from protoplasts

Details Method of regenerating rice plant from protoplasts Method of regenerating rice plant from protoplasts

A - Human Necessities

01

H

47/4, 195/47

A01H 4/00 (2006.01) C12N 5/04 (2006.01)

Patent

CA 1288713

- 23 - ABSTRACT OF THE DISCLOSURE Method of generating a complete plant of rice from a protoplast thereof. To prepare the protoplast, a callus is derived from an embryo, scutellum, or radicle of a seed of rice. The callus is then cultured in a liquid medium to form a small cell cluster which readily releases a protoplast. The cell cluster is then treated with a protoplast-releasing enzymatic solution to obtain a protoplast. The protoplast is then cultured to form a callus, and the callus is then cultured in a differentiation medium to regenerate a whole plant of rice.

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