Method of regulating expression of a foreign gene by...

Details Method of regulating expression of a foreign gene by... Method of regulating expression of a foreign gene by...

C - Chemistry, Metallurgy

12

N

195/1.19, 195/1.

C12N 15/60 (2006.01) C12N 9/88 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1320922

Abstract of the Disclosure Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured at a temperature 40°C or over so that the expression of said desired foreign gene was suppressed. This E. coli (i.e., transformant) was cultured at 40°C or over in a first process to suppress the expression of the foreign gene and to support sufficient cell growth and thereafter below 40°C in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture. 56

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