C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.2, 195/1.2
C12N 15/60 (2006.01) C12N 9/88 (2006.01) C12N 15/70 (2006.01)
Patent
CA 1319630
Abstract of the Disclosure Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coll (i.e., transformant) was cultured in the medium in which the sugar concentration was maintained at 0.3%or more in a first process so as to supress the suppression of the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture. 57
559122
Fukuhara Nobuhiro
Makiguchi Nobuyoshi
Nakajima Yoshiyuki
Sone Satori
Yoshino Setsuo
Mitsui Chemicals Incorporated
Sim & Mcburney
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