Method of removing hepatitis-associated antigen from protein...

A - Human Necessities – 61 – K

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A61K 39/12 (2006.01) A61K 35/16 (2006.01) C07K 14/02 (2006.01)

Patent

CA 1061251

ABSTRACT OF THE DISCLOSURE Hepatitis-associated antigen is removed from a protein fraction containing same by solubilizing the protein in a sol- vent physiologically tolerable on injection, e.g., glycine- citrate-saline buffer; adding polyethylene glycol having a molecular weight of from about 200 to about 6,000 to a concen- tration of from about 12 to about 30 grams per 100 ml of solution to yield a precipitate and separating the precipitate. The protein fraction may be, for example, albumin, gamma globulin, coagulation factors II, V, VII, IX, X, XI and XIII, fibrinogen or plasminogen.

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