Method of removing lipid inhibitors from limulus amebocyte...

Details Method of removing lipid inhibitors from limulus amebocyte... Method of removing lipid inhibitors from limulus amebocyte...

G - Physics

01

N

195/77, 195/86

G01N 33/48 (2006.01) G01N 33/579 (2006.01)

Patent

CA 1149300

ABSTRACT OF THE DISCLOSURE The improved method of the present invention involves the removal of lipid inhibitors from Limulus amebocyte lysate to increase the sensitivity and quality of the lysate. The method comprises intimately contacting, as by mixing and stirring, Limulus amebocyte lysate with a selected binary liquid solvent system for a time sufficient to draw water into the solvent system from the lysate and to effectively extract and denature the lipid inhibitors in the lysate. The ability of the binary solvent to draw in water from the lysate and form, in effect, a new tertiary system is the important factor in this methodology. The method of water drawn into the solvent is controlled by the amount of polar solvent in the solvent system. After this extraction and denaturation, the lysate is then separated from the solvent system and recovered in purified from of increase sensitivity.

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