C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/134, 195/1.2
C12P 21/00 (2006.01) C07K 14/555 (2006.01) C07K 14/61 (2006.01) C12N 9/48 (2006.01) C12P 21/06 (2006.01)
Patent
CA 1318869
METHOD OF REMOVING N-TERMINAL AMINO ACID RESIDUES FROM EUCARYOTIC POLYPEPTIDE ANALOGS AND POLYPEPTIDES PRODUCED THEREBY Abstract of the Disclosure A method of sequentially removing one or more N-terminal amino acid residues from an analog of a eucaryotic poly- peptide synthesized in a foreign host, comprises contacting the eucaryotic polypeptide analog with aminopeptidase under suitable conditions permitting sequential removal of N- terminal amino acid residues, where the polypeptide analog contains an amino acid residue or sequence of residues which blocks the action of an aminopeptidase located at a position other than the N-terminal end of the polypeptide analog. A specific embodiment of the invention concerns removing both an N-terminal methionine residue and its adjacent Leucine residue from a growth hormone analog produced in bacteria by contacting the growth hormone analog with Aeromonas aminopeptidase under suitable conditions per- mitting removal of the N-terminal methionine and Leucine residues. The invention also concerns eucaryotic poly- peptide analogs produced in accordance with the methods of the invention.
488771
Blumberg Shmaryahu
Meir Daniela Ben
Gowling Lafleur Henderson Llp
Savient Pharmaceuticals Inc.
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