C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C07K 14/00 (2006.01) C12N 9/12 (2006.01) C12P 19/34 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2401732
The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
La présente invention concerne la transcription inverse de l'ARN, et en particulier la transcription inverse par ADN polymérases thermostables. Le <i>Thermoactinomyces vulgaris</i> et le <i>Bacillus stearothermophilus</i> possèdent une activité de transcriptase inverse en présence d'ions de magnésium ou de manganèse. L'invention concerne également des méthodes, des compositions et des kits de transcription inverse et RT-PCR.
Gu Trent
Hartnett James Robert
Huang Fen
Promega Corporation
Smart & Biggar
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