Method of sample preparation for urine protein analysis with...

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G01N 33/493 (2006.01) G01N 1/34 (2006.01) G01N 27/447 (2006.01) G01N 30/14 (2006.01) G01N 33/483 (2006.01) G01N 33/49 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2143206

Processes are provided for pretreating body fluid compositions and subsequently analyzing the pretreated body fluid compositions for analytes of interest. Processes for penetrating the compositions include providing size exclusion gel having a molecular weight fractionation range or a molecular weight exclusion such that the size exclusion gel is capable of excluding or fractionating the analytes of interest, and then causing the composition to contact the size exclusion gel in order to separate the analytes from low molecular weight composition components which interfere with the separation and analysis of the analytes of interest. Processes for analyzing pretreated compositions include electrophoretic methods such as capillary zone electrophoresis which involve the separation and detection of analytes of interest.

Procédé de prétraitement de compositions à base de fluides biologiques et d'analyse ultérieure desdites compositions afin d'y détecter des analytes à étudier. Des procédés de prétraitement des compositions consistent à utiliser un gel d'exclusion présentant une plage de fractionnement de poids moléculaire ou une exclusion de poids moléculaire lui permettant d'exclure ou de fractionner lesdits analytes à étudier, puis à mettre la composition en contact avec le gel d'exclusion afin de séparer les analytes des constituants de faible poids moléculaire de la composition qui perturbent la séparation et l'analyse desdits analytes. Des procédés d'analyse des compositions prétraitées comprennent des procédés électrophorétiques tel que l'électrophorèse de zone par colonne capillaire qui impliquent la séparation et la détection des analytes à étudier.

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