Method of screening for enzyme activity

Details Method of screening for enzyme activity Method of screening for enzyme activity

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 9/16 (2006.01) C12N 9/24 (2006.01) C12N 15/10 (2006.01) C12Q 1/25 (2006.01)

Patent

CA 2239686

Disclosed are processes to identify desired enzymatic activity from a pool of DNA collected from one or more organisms or a DNA subjected to random directed mutagenesis. The methods involve the generation of DNA library in a host cell and screening for the desired activity. The process can be applied to develop thermally stable proteins having improved enzymatic activity at lower temperature.

L'invention concerne des procédés d'identification d'une activité enzymatique voulue dans un ensemble d'ADN provenant d'un ou de plusieurs organismes ou bien à partir d'un ADN soumis à une mutagénèse aléatoire dirigée. Les procédés consistent à produire une banque d'ADN dans une cellule hôte et à détecter l'activité voulue. Le procédé peut être appliqué pour développer des protéines stables thermiquement présentant une activité enzymatique améliorée à des températures plus basses.

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