Method of screening for improved specific activity of enzymes

Details Method of screening for improved specific activity of enzymes Method of screening for improved specific activity of enzymes

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2531494

This invention relates to a method for screening libraries of enzyme variants for changes in specific activity by expression of a fusion protein consisting of at least two enzymes. By using one enzyme as a marker changes in specific activity for the other enzyme can be screened efficiently.

L'invention se rapport à un procédé de criblage de banques de variants enzymatiques aux fins de la détection de modifications de l'activité spécifique par expression d'une protéine de fusion constituée d'au moins deux enzymes. En utilisant une enzyme en tant que marqueur, il est possible de détecter par criblage, de manière efficace, des modifications de l'activité spécifique de l'autre enzyme.

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