Method of screening for protein inhibitors and activators

Details Method of screening for protein inhibitors and activators Method of screening for protein inhibitors and activators

C - Chemistry, Metallurgy

12

Q

167/45, 150/8

C12Q 1/02 (2006.01) C12N 9/12 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/554 (2006.01)

Patent

CA 1334927

This invention relates to a general screening method for the discovery and identification of both inhibitors and activators of enzymes, receptors, and other proteins. In particular, it is concerned with a method of screening for substances which specifically inhibit or activate a particular protein affecting the cultural or morphological characteristics of the cell expressing the protein, especially in a manner apparent to the naked eye. In brief, the method which we describe herein involves the generation of a cell line purposefully engineered to detect both stimulatory and inhibitory agents which are absolutely specific for any given protein which affects the cultural or morphological characteristics of the cell. The basis for this invention is my observation that if a protein (the "protein of interest", or POI) which is involved in some manner in cellular growth control is overproduced in cells, then pharmacologic agents which can activate or inhibit the POI can result in altered growth properties of the cells. The sensitivity of the cells is dependent on their production of the POI, a phenomenon referred to herein as a "graded cellular response" to the pharmacologically active agent. By this method, tamoxlfen is identified as an inhibitor of PKC activity in cell culture.

590540

Affiliated with

Also associated with

Rating

Method of screening for protein inhibitors and activators does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of screening for protein inhibitors and activators, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of screening for protein inhibitors and activators will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1188484