Method of screening reprogramming factor, reprogramming...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/02 (2006.01) A01K 67/027 (2006.01) A61K 35/12 (2006.01) A61L 27/00 (2006.01) A61L 27/38 (2006.01) C12N 5/16 (2006.01) C12N 15/02 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2461185

Somatic cells are treated with a component contained in ES cells and the activity is detected. Thus, a reprogramming factor can be screened. Moreover, somatic cells can be reprogrammed by treating with a component containing the reprogramming factor or the reprogramming factor per se. Since the tetraploid cells as described above are capable of undergoing proliferation and multidifferentiation, cells, tissues and organs usable in transplantation are produced by differentiating the cells.

Selon l'invention, des cellules somatiques sont traitées au moyen d'un composé contenu dans des cellules ES et leur activité est détectée. Ainsi, un facteur de reprogrammation peut être criblé. En outre, des cellules somatiques peuvent être reprogrammées par traitement au moyen d'un composé contenant le facteur de reprogrammation ou le facteur de reprogrammation per se. Etant donné que les cellules tétraploïdes décrites ci-dessus peuvent être soumises à la prolifération et à la multidifférenciation, des cellules, des tissus et des organes pouvant être utilisés dans la transplantation sont produits par différenciation de cellules.

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