Method of selecting a lipolytic enzyme

Details Method of selecting a lipolytic enzyme Method of selecting a lipolytic enzyme

C - Chemistry, Metallurgy

12

Q

C12Q 1/44 (2006.01) A21D 8/04 (2006.01) C12N 9/18 (2006.01) C12N 9/20 (2006.01)

Patent

CA 2508812

A method of screening lipolytic enzymes is used to identify candidates for a baking additive which can improve the properties of a baked product when added to the dough. The screening method involves contacting the enzyme with N-acyl phosphatidyl ethanolamine (APE) or N-acyl lyso-phosphatidyl ethanolamine (ALPE), and detecting hydrolysis of an ester bond in the APE or ALPE.

Un procédé de criblage d'enzymes lipolytiques est utilisé pour l'identification de candidats pour un additif de cuisson pouvant améliorer les propriétés d'un produit cuit au four lorsqu'il est ajouté à la pâte. Le procédé de criblage consiste à faire incuber l'enzyme avec de la N-acyl phosphatidyl éthanolamine (APE) ou de la N-acyl lyso-phosphatidyl éthanolamine (ALPE), et à détecter l'hydrolyse d'une liaison ester dans APE ou ALPE.

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