Method of selecting a population or subpopulation of a...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/24 (2006.01) C12Q 1/04 (2006.01) G01N 33/537 (2006.01) G01N 33/553 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2237119

A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles (14) have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample (12) by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernantant can be further enriched by multiple removal steps.

Procédé de séparation permettant de séparer une population désirée ou indésirable d'un échantillon biologique à l'aide de particules denses relativement lourdes et de la sédimentation gravimétrique. Les particules (14) portent fixés sur elles un ou plusieurs réactants spécifiques de la population sélectionnée, qui vont se fixer sur celle-ci. De préférence, on mélange les particules à l'échantillon (12) par sédimentation répétée dans une partie substantielle de l'échantillon, de façon à les fixer sur la population sélectionnée. Puis on laisse les particules fixées à la population sélectionnée se déposer de façon préférentielle dans l'échantillon, et on sépare des particules portant fixées sur elles la population sélectionnée le surnageant contenant une population enrichie. On peut ensuite, par de multiples étapes supplémentaires, continuer à enrichir les populations déjà enrichies contenues dans le surnageant biologique.

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