Method of selecting recombinant dna-containing streptomyces

Details Method of selecting recombinant dna-containing streptomyces Method of selecting recombinant dna-containing streptomyces

C - Chemistry, Metallurgy

12

N

195/1.18, 195/1.

C12N 15/00 (2006.01) C12N 1/20 (2006.01) C12N 15/65 (2006.01) C12N 15/76 (2006.01)

Patent

CA 1266242

Abstract A novel method of selecting Streptomyces recombinant DNA-containing host cells and vectors useful in the method are described. The vectors confer tylosin resistance to sensitive Streptomyces host cells and thus provide a convenient method of selecting Streptomyces transformants. The novel tylosin resistance-conferring gene described can be isolated on an ?2.6 kb KpnI restriction fragment from plasmid pSVB2. Plasmid pSVB2 can be isolated from Streptomyces lividans TK23/pSVB2 (NRRL 15880).

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