Method of selection of a population or subpopulation of a...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/08 (2006.01) B01D 21/00 (2006.01) C12M 3/00 (2006.01) C12N 5/06 (2006.01) G01N 33/543 (2006.01) G01N 33/553 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2187543

A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including the non-selected population is separated from the particles with the selected population bound thereto. The particles can be heated for sterilization and endotoxin removal.

Est décrit un mode opératoire permettant de séparer une population désirée choisie et une population non souhaitée dans un échantillon biologique à l'aide de particules denses relativement lourdes et d'une sédimentation par gravité. Ces particules sont liées à un ou plusieurs réactants qui sont spécifiques de la population choisie et vont s'y fixer. Ces particules sont mélangées de préférence avec l'échantillon par décantation répétée à travers une partie substantielle de l'échantillon en vue de leur fixation sur la population choisie. On laisse ensuite les particules ainsi que la population choisie fixée se décanter de préférence dans l'échantillon, et le surnageant renfermant la population non choisie est séparé des particules ainsi que de la population choisie qui s'y est fixée. Les particules peuvent être chauffées en vue de leur stérilisation et de l'élimination des endotoxines.

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